At 37, without (lane 1) or with (lane 2) of IPTG (1 mM), LB medium for 3 hours, then the amount of crude extracts with 7.5% SDS-page separation and Coomassie blue staining.
At 37 ℃, without (lane 1) or containing (lane 2) IPTG (1 mM), cultured in LB liquid medium for 3 hours and then the amount of bacteria and other proteins in the crude extract was separated 7.5% SDS-PAGE, with Coomassie and blue staining.
At 37 C, excluding (Lane 1) or lane (2) IPTG (1 mM), in LB liquid culture medium for 3 hours, then the amount of the protein crude extract was separated by 7.5% SDS-PAGE, and blue Coomassie staining was used.