Biotin
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Biotin acts in certain metabolic reactions as an intermediate carrier of carbon dioxide during carboxylation and decarboxylation reactions.
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Specific enzymes that require biotin include acetyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase. Metabolic pathways requiring biotin include the biosynthesis of long-chain fatty acids and the synthesis of purines.