The Swiss female mice received an intraperitoneal injection of 0.5 mL of 1% (w/v) carrageenan. After 4 h, the animals were euthanized with a high dose of pentobarbital (120 mg/kg, i.p.) and the peritoneal cavity was washed with 2 mL ofphosphate-buffered saline (PBS), pH 7.6, containing 2 mM EDTA. The peritoneal fluid was collected, and the total leukocyte count was determined on the ABX Micros analyzer. The experiments were also approved by the Ethics Committee of the Universidade Federal de Pernambuco (no. 23076.025944/2018-49).
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