Transmission electron microscopy (TEM), nanoparticle<br>tracking analysis (NTA) technology, and Western blotting<br>(WB) was employed to comprehensively outofthe the<br>Particles derived from PRP, called PRP-Exos. TEM clearly<br>revealed it PRP-Exos exhibited a round-shaped morphology<br>(Fig. 1a), and NTA showed that the majority of<br>PRP-Exos had a similar size of 145.6 s 50.4 nm (Fig. 1c).<br>Additionally, Western Blotting (Fig. 1b) confirmed that<br>PRP can secrete exosomes that expressed ed<br>exosomal surface markers, such as CD9, CD63, CD81,<br>and HSP101. All data indicated ed it the nanoparticles derived<br>From PRP was PRP-Exos.
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