Polar leachate preparation: sterile operation, take the catheter (remove the tube body protective sleeve, seal cap, slip buckle) 5.5g, according to the ratio of 0.2g/mL into the immersion medium 27.5mL, 37 degrees C under the placement of 72h old people's products ready leaching liquid.<br>Grade, Breed: SPF-class KM mice<br>Animal reasons were selected in the trial: mice have a long history of application and rich background in evaluating the risk of acute systemic toxicity.<br>International and national standards recommend the use of mice for acute systemic toxicity testing to assess the potential risk of acute systemic toxicity of test materials in the test criteria.<br>Species: Mice maintained active coordination<br>Pads<br>Type: Poplar wood mats, after sterilization<br>Type: Purified water after sterilization in both countries<br>Water supply: free water through drinking water bottles<br>Electronic balance C2006-10-04 (D), biochemical culture box T2016-10-20 (D), biosecurity cabinet Z2015-25-11 (D)<br>Pre-test preparation: take 20 mice, using 5% bitter acid solution dyeing method to mark, randomly divided into 4 groups, each group of 5, and weigh.<br>Test procedure: 10 mice per leachate, for use in the trial group and leaching medium control two groups, each group of 5.<br>Tail intravenous injection 2 groups, respectively, injected with physiological saline leachate and physiological saline negative control fluid, injection dose of 50mL/kg, injection speed is not percentage of 2 groups, injection speed of 2 groups, respectively, injected cottonseed oil leachate and cottonseed oil negative control fluid, injection dose of 50mL/kg.<br>Results Observation: Immediate observation of mouse response after injection, and in 4h, 24h, 48h and 72h test observation groups and control groups of the general state, toxic performance and number of dead animals and animal weight, animals appear clinical symptoms and observation items are shown in the table below:<br>Common clinical symptoms and observation items<br>Results evaluation<br>Animal acute systemic toxicity reactions are judged according to the following criteria:
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