我第一次接觸到細胞生物學是在大三的時候，這門課的主要內容分成細胞組成、

我第一次接觸到細胞生物學是在大三的時候，這門課的主要內容分成細胞組成、基因與分子的調控、細胞訊息傳遞作用、細胞功能缺失與疾病的產生等四的部分。給我留下特別深刻的印象的是，儘管技術有隨著時代而進步，但是關於生物科技的部分其實還有許多知識是我們不清楚的。與此同時，因為進入實驗室為我們的必修學分之一，我也因此進入了細胞實驗室。剛開始的時候我只有負責讀paper並報告而已，畢竟還不是那麼確定自己想進入這個產業，但後來隨著聽了越來越多位實驗室學長姊的實驗報告後，發現自己也對做實驗滿有興趣的，為什麼會發生癌症？中間的機制是什麼？我該怎麼設計我的實驗？諸如此類的問題都令我著迷，因此在跟老師討論過後，我開始了我的實驗生活。不管第一次養細胞、第一次做western、第一次做wound healing 都讓我興奮不已，就算做實驗做到很晚，我還是樂在其中。大四時，我也開始進行了我的大學論文，第一次有自己的project令我覺得非常興奮也很驕傲，我被分配的實驗是針對肝癌的部分，發現的問題是科學家發現目前肝癌對於市售藥物Sorafenib有抗藥性，因此我的研究就是朝其中的機制去找尋突破點，查找paper後發現到有可能是因為經過Sorafenib 處理後，細胞磷酸化的FAK分子表達會明顯上升，而FAK分子會跟Src互相磷酸化，進而促使下游分子的訊息傳遞並促進細胞增生及爬行，因而產生了抗藥性。而我的論文就是在講述透過結合Sorafenib和Src抑制劑-Dasatinib 來看是否能抑制HCC細胞株HepG2的細胞增生，進而解決Sorafenib的抗藥性問題，結果發現藥物處裡可以顯著抑制細胞存活率卻無法抑制其增生，但這到底是什麼原因造成的我們也不知道，只能說其中的作用機制仍待研究。在這裡要特別感謝實驗室的老師及學長姐們，不厭其煩的讓我問問題、討論並給予我指導。

結果 (英文) 1:

I was first exposed to the cell biology in junior when this course the main content is divided into cells, gene regulation and molecular, cellular messaging, cell loss of function generating portion and four other diseases. I am particularly impressed that, despite the technical advances with the times there, but the section on biotechnology there is still much we do not know the knowledge is. At the same time, because enter the laboratory is one of our compulsory credits, and thus I entered the cell laboratory. At first I only read the paper and is responsible for reporting it, after all, is not so sure they want to enter this industry, but then after listening to more and more as the number of laboratory experiments percent of seniors report, found himself on doing experiments with interest, why cancer occurs? What the middle of the mechanism? How can I design my experiment? Questions like these have fascinated me, so after a discussion with the teacher, I started my experiment life. Regardless of the first support cells, the first time for western, the first time for wound healing makes me excited, even if doing experiments done late, I still enjoy it. Senior year, I started my college paper, for the first time have their own project makes me feel very excited and very proud, I was assigned for the experiment is part of liver cancer, scientists have found that the problems identified are currently for liver cancer Sorafenib marketed drug resistant, so my research is the mechanism towards which to find the breakthrough point, to look after the paper found that it may be because the Sorafenib treatment, the intracellular phosphorylation of FAK molecule expression will be significantly increased, while the FAK molecules phosphorylation with each other will Src, thereby promoting the message is transmitted downstream molecules and promotes cell proliferation and creeping, thereby generating a resistance. And my thesis is that through a combination of telling whether it can inhibit cell proliferation of Sorafenib HCC cell lines HepG2 and Src inhibitor -Dasatinib point of view, and then solve the problem of drug resistance Sorafenib, and found drugs at significantly inhibited cell viability was possible but can not inhibit their proliferation, but in the end is what causes we do not know, I can only say that the mechanism of action remains to study them. Here special thanks to the teachers and seniors laboratory sister who patiently let me ask questions, discuss and give me guidance.

結果 (英文) 2:

My first exposure to cell biology was in my junior year, when the main content of this course was divided into four parts: cell composition, gene and molecular regulation, cell messaging, cell function loss and disease generation. I was particularly impressed that although technology has evolved with the times, there is a lot of knowledge about biotechnology that we don't know. At the same time, because entering the lab is one of our required students, I also entered the cell lab. At first I was only responsible for reading paper and reporting, after all, not so sure that I want to enter the industry, but then after listening to more and more laboratory students and sister's experimental report, found that they are also full of interest in doing experiments, why did cancer occur? What is the intermediate mechanism? How do I design my experiment? I was fascinated by such questions, so after discussing them with my teacher, I began my experimental life. Whether it's the first cell, the first time i do western, the first time I do wound healing all make me excited, even if i do the experiment late, I still enjoy it. In my freshman year, I also started my college thesis, the first time I had my own project makes me very excited and proud, I was assigned the experiment for liver cancer part, found that the problem is that scientists found that liver cancer is currently resistant to the commercial drug Sorafenib, So my research is looking for a breakthrough point in the mechanism, looking for paper found that it is possible that after Sorafenib treatment, cell phosphorylation of FAK molecular expression will increase significantly, and FAK molecules and Src phosphorylation, In turn, downstream molecules are sent through and cell proliferation and crawling are promoted, resulting in resistance. And my paper is on whether the cell growth of HCC cell line HepG2 can be inhibited by combining Sorafenib and Src inhibitor-Dasatinib to solve Sorafenib's resistance problem. It was found that the drug's ability to significantly inhibit cell survival but not its growth, but we do not know exactly what causes it, only that the mechanism of action is still to be studied. Here to thank the lab teachers and sisters, i am tired of asking questions, discussion and give me guidance.

結果 (英文) 3:

When I first came into contact with cell biology in my junior year, the main content of this course is divided into four parts: cell composition, gene and molecular regulation, cell information transmission, cell function loss and disease generation. What impresses me is that although technology has progressed with the times, there are still many knowledge about biotechnology Knowledge is not clear to us. At the same time, because entering the laboratory is one of our compulsory credits, I also entered the cell laboratory. At the beginning, I was only responsible for reading paper and reporting. After all, I was not so sure that I wanted to enter the industry, but later, after listening to more and more lab elder sisters' experimental reports, I found that I also did the same The experiment is full of interest. Why does cancer happen? What's the mechanism in the middle? How can I design my experiment? All these questions fascinate me, so after discussing with the teacher, I started my experimental life. No matter the first cell culture, the first western, the first round healing I'm very excited. Even if I do the experiment very late, I still enjoy it. In my senior year, I also started my university thesis. I felt very excited and proud to have my own project for the first time. The experiment I was assigned was aimed at the part of liver cancer. The problem was that scientists found that the liver cancer was resistant to sorafenib, which is a drug sold on the market. So my research It's to find a breakthrough point in the mechanism. After searching for paper, it's possible that it's because of sorafenib After treatment, the expression of phosphorylated FAK molecules will increase significantly, and FAK molecules and Src will phosphorylate each other, which will promote the information transmission of downstream molecules and promote cell proliferation and crawling, thus resulting in drug resistance. My paper is about the combination of sorafenib and Src inhibitor dasatinib To see whether it can inhibit the proliferation of HepG2 cell line of HCC, and then solve the problem of sorafenib resistance, it was found that the drug can significantly inhibit the cell survival rate, but it can't inhibit the proliferation, but we don't know what the cause is, only to say that the mechanism of action remains to be studied. Thank the teachers and students of the laboratory in particular, No I am tired of asking questions, discussing and giving guidance.