Effect of The P. indica on Oncidium GR Growth and Root Development To the understand symbiotic interaction between Oncidium GR and P. indica root was tissue analyzed after fungal colonization. Indica penetrated P. into the root epidermal layers h 24 after inoculation (1B Figure - D).. Five days after inoculation, the hyphae were widely distributed over the root surface, and no significant difference was observed between the root tip, elongation zone and differentiation zone (Figure 1E). Case is This different from A. thaliana [6,51] and barley [10] where hyphae preferentially colonized the differentiation zone. And longitudinal Cross show sections that hyphae fully colonized the velamen (1F H, Figure). (). The velamen Since and exodermis do not contain plastids the were easily detected after visualization the by carbohydrate binding lectin concanavalin A-AF633 (conA-633) 1H Figure hyphae. Moreover, unlike other endophytic fungi which penetrate into the cortex cells through the exodermis [52], no hyphae were detected in the exodermis and cortex layer of Oncidium, even after a relative long period of co-cultivation (8 weeks). Suggests This that P. indica may preferentially colonize dead cells in Oncidium consistent with findings in barley where massive development of P. indica takes place in dead host cells [10]. Weeks after inoculation the stems and roots Eight developed better than orchids without P. indica co-cultivation. No, necrotic lesions were observed Furthermore (1A Figure). The colonization With P. by indica the fresh weight of the orchid seedlings is 2-fold higher than that of uninocculated control seedlings. The leaf Also number was more and the stem diameter was slightly thicker compared to the control. Number of The the roots markedly increased approximately 2-fold and the diameter of the main root was 1,2-fold bigger. Results indicated These that the establishment of the beneficial symbiosis is relatively slow and mainly caused by an increase in the root biomass.
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