During the experiment, only one A. hyacinthus was observed producing mesenterial filaments, and therefore the analysis focused on mesenterial filaments extruded by G. fascicularis, each of which released multiple mesenterial filaments throughout the experiment. For G. fascicularis, the extrusion of mesenterial filament differed over time (Pseudo-F5,10 = 4.14, p = 0.032), and the effect of time differed between pCO2 treatments (i.e., the time × pCO2 interaction was significant: Pseudo-F5,10 = 3.64, p = 0.043). This interactive effect was noticeable on the 4th day of the incubation, when G. fascicularis at elevated pCO2 extruded 5–15 mesenterial filament polyp−1 (with each filament ~5-mm long), while none was observed for polyps at ambient treatment pCO2 until day 6 (Fig. 3). From day 6 onwards, G. fascicularis under ambient pCO2 also extruded 5–15 mesenterial filament polyp−1. Thereafter, mesenterial filament extrusion decreased in both treatments as parts of the adjacent A. hyacinthus colonies were killed by mesenterial filament contact. Overall, there was no statistically significant effect of pCO2 on mesenterial filament extrusion (Pseudo-F1,2 = 7.20, p = 0.346), despite more mesenterial filaments extruded at elevated pCO2 ( 2.83 ± 0.58 mesenterial filament polyp−1) than ambient pCO2 (1.83 ± 0.23 mesenterial filament polyp−1m ; n = 4).
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