Thirteen healthy, thin-skinned European breed heifers from a diverse genetic pool were sourced from herds where LSD had not been seen for three years, and where no vaccination was practiced. Only animals that tested negative for antibodies to LSDV by using the serum neutralization test (SNT) were selected and kept at the insectfree stables of the Biological Research Centre of the University of Pretoria (UPBRC), Faculty of Veterinary Science.
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