Aging is is a degenerative cheded ed by a progressive sylwedd of cellular components and organelles in-mortality. The budding yeast Saccharomycecece ceisiaeth has had ei ed dedi to study the biology of, and ahasigs of the glyns of yeast longe have hadhas has shown to be yn multicellular eukaryotes, including worms, flies, and mice. Due to the lack of easilyed age-associated phenotype, aging in yeast has has been assayed almost exclusive ly by-the-life the life span of in different context, with two life span paradigms in common usage. Chronological life span refers to the to the time of the time of the time of the one-in-one-time a mother-cell can-in-a non-dividing, quiescence-like state, and is-proposed to serve as a model for aging of the post-mitotic cells in multicellular eukaryotes. Replicative life span, in contrast, the number of the number of daughter cells by-a-mother mother pre to senscence, and is-thought to provide a model of ingestion in mitotically active cells. Here we a generalized protocol for chylin dynth i'r siaspan of budding yeast mother cells. The goal of the insatilife life span assay is to how how many times each sylcell buds. The mother and saly cells can easily ared by an experienced researcher a standard light microscope (total magnification 160X), such as the Zeiss Axio ath4 or comparable model. Physical separation of salys from s mother mother cells is dedd using a manual micromanipulator dywedodd a fiber-optic needle. Typical laboratory yeast strains produce 20-30 daughter cells per mother and one life span experiment requires 2-3 weeks.
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